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KMID : 0363420090230030009
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Journal of Korean Oriental Pediatrics
2009 Volume.23 No. 3 p.9 ~ p.36
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Effects of Concurrent Administration of JaUmJeSeupTangKaKam(JUJSTK) and Atopy Cream, Jawoongo(AJ) on Atopic Dermatitis-like Skin Lesions in NC/Nga Mouse
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Lee Nam-Yerl
Kim Yun-Hee
Han Jae-Kyung
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Abstract
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Objectives: The purpose of this study is to examine the effect of a concurrent administration of JUJSTK and AJ on atopic dermatitis in an in-vivo experiment. Thus, this study is expressed by using NC/Nga atopic dermatitis mice which have histological and clinical similarities to that of humans have been used.
Methods: Clinical skin score, hematology, serum total IgE and IgG1 of the mouse was evaluated, and cytokine levels, total number of the cells, immunohistochemical staining, histological features of axillary lymph node(ALN), peripheral blood mononuclear cells(PBMCs), and a dorsal skin tissue of the mouse were analyzed.
Results: Oral administration of JUJSTK and concurrent administration of JUJSTK and AJ lowered the clinical skin score, total cell number of WBC, eosinophils in blood, and serum total of IgE & IgG1, IFN-γ, IL-5, IL-13, IL-17. In addition, total cell number of ALN and dorsal skin tissue, absolute cell number of CD3e+ T cell, CD4+ Th cell, CD8+ c/sT cell, CD3+CCR3+ cell, CCR3+ cell, CD3+CD69+, CD4+CXCR5+ in ALN, PBMCs, absolute cell number of CCR3+, CD3+/CD69+, CD11b+/Gr-1+, CD11b+/Gr-1+ in dorsal skin tissue, Eotaxin2 mRNA, CCR3 mRNA in dorsal skin tissue and gene expression of IL-5 mRNA, IL-13 mRNA in ALN were significantly decreased. Furthermore, thickness of epidermis infiltrated inflammatory immune cell & mast cell in dermis, histological infiltration of mast cell, the size of inflammatory lymphocytes cells & plasma cells in ALN and histological infiltration of CD4+ & CCR3+in ALN and dorsal skin tissue were significantly decreased as well.
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KEYWORD
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atopic dermatitis, JaUmJeSeupTangKaKam, atopy cream, Jawoongo, NC/Nga mouse
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